Western blotting analysis using CAMK2D antibody (Cat#71142). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAMK2D antibody (Cat#71142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CAMK2D antibody (Cat#71142). CAMK2D expression in wild type (WT) and CAMK2D knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CAMK2D antibody (Cat#71142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CAMK2D expression in C2C12 cells using CAMK2D antibody (Cat# 71142, 1:2,000). Green, isotype control; red, CAMK2D.
CAMK2D; Calcium/Calmodulin Dependent Protein Kinase II Delta;CAMKD; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Delta; Calcium/Calmodulin-Dependent Protein Kinase Type II Subunit Delta; EC 2.7.11.17; Calcium/Calmodulin-Dependent Protein Kinase Type II Delta Chain; CaM Kinase II Delta Subunit; CaM Kinase II Subunit Delta; CaM-Kinase II Delta Chain; CaMK-II Delta Subunit; CaMK-II Subunit Delta; EC 2.7.11
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human CaMKII delta
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using CAMK2D antibody (Cat#71142). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAMK2D antibody (Cat#71142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CAMK2D antibody (Cat#71142). CAMK2D expression in wild type (WT) and CAMK2D knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CAMK2D antibody (Cat#71142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CAMK2D expression in C2C12 cells using CAMK2D antibody (Cat# 71142, 1:2,000). Green, isotype control; red, CAMK2D.
Western blotting analysis using CAMK2D antibody (Cat#71142). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAMK2D antibody (Cat#71142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CAMK2D antibody (Cat#71142). CAMK2D expression in wild type (WT) and CAMK2D knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CAMK2D antibody (Cat#71142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CAMK2D expression in C2C12 cells using CAMK2D antibody (Cat# 71142, 1:2,000). Green, isotype control; red, CAMK2D.
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