Western blotting analysis using tank binding kinase 1 antibody (Cat#71180). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tank binding kinase 1 antibody (Cat#71180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using tank binding kinase 1 antibody (Cat#71180). TANK binding kinase 1 expression in wild-type (WT) and tank binding kinase 1 (TBK1) knockout (KO) 4T1 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with tank binding kinase 1 antibody (Cat#71180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human melanoma using tank binding kinase 1 antibody (Cat#71180, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using tank binding kinase 1 antibody (Cat#71180, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse spleen using tank binding kinase 1 antibody (Cat#71180, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of tank binding kinase 1 expression in HepG2 cells using tank binding kinase 1 antibody (Cat#71180, 1:2,000). Green, isotype control; red, tank binding kinase 1.
