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Integrin Subunit Alpha 4 Recombinant Rabbit mAb

#1316

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Recombinant Antibody
Western blotting analysis using Integrin subunit alpha 4 antibody (Cat#1316). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin subunit alpha 4 antibody (Cat#1316, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
Flow cytometric analysis of Integrin subunit alpha 4 expression in HAP-1 cells using Integrin subunit alpha 4 antibody (Cat#1316, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Integrin subunit alpha 4.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ITGA4
  • Catalog:
    1316
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name Integrin Subunit Alpha 4 Recombinant Rabbit mAb
Aliases ITGA4; Integrin Subunit Alpha 4; CD49D; Alpha 4 Subunit Of VLA-4 Receptor; CD49 Antigen-Like Family Member D; VLA-4 Subunit Alpha; Integrin Alpha-IV; Integrin Alpha-4; Integrin, Alpha 4 (Antigen CD49D, Alpha 4 Subunit Of VLA-4 Receptor); Very Late Activation Protein 4 Receptor, Alpha 4 Subunit; Antigen CD49D, Alpha-4 Subunit Of VLA-4 Receptor; 269C Wild Type; Antigen CD49D; CD49d Antigen; CD49d; IA4
Background

Gene Name: ITGA4

NCBI Gene Entry: 3676

UniProt Entry: P13612

Application Information

Molecular Weight: Predicted, 115 kDa; observed, 150 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2480

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Integrin alpha 4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using Integrin subunit alpha 4 antibody (Cat#1316). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin subunit alpha 4 antibody (Cat#1316, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
  • Flow cytometric analysis of Integrin subunit alpha 4 expression in HAP-1 cells using Integrin subunit alpha 4 antibody (Cat#1316, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Integrin subunit alpha 4.
Western blotting analysis using Integrin subunit alpha 4 antibody (Cat#1316). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin subunit alpha 4 antibody (Cat#1316, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
Flow cytometric analysis of Integrin subunit alpha 4 expression in HAP-1 cells using Integrin subunit alpha 4 antibody (Cat#1316, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Integrin subunit alpha 4.
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