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Home>Product>Primary Antibody

Phospho-PLK1 (T210) Recombinant Rabbit mAb

#1690

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Recombinant Antibody
Western blotting analysis using Phospho-PLK1 (T210) antibody (Cat#1690). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PLK1 (T210) antibody (Cat#1690, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Phospho-PLK1 (T210) antibody (Cat#1690, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PLK1 (T210) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PLK1
  • Catalog：
    1690
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name Phospho-PLK1 (T210) Recombinant Rabbit mAb
Aliases PLK1; Polo Like Kinase 1; PLK; Serine/Threonine-Protein Kinase PLK1; Serine/Threonine-Protein Kinase 13; Polo-Like Kinase 1; EC 2.7.11.21; STPK13; PLK-1; Cell Cycle Regulated Protein Kinase; Polo-Like Kinase (Drosophila); Polo (Drosophia)-Like Kinase; EC 2.7.11
Background

Gene Name: PLK1

NCBI Gene Entry: 5347

UniProt Entry: P53350

Application Information

Molecular Weight: Predicted, 68 kDa; observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4090

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-PLK1 (T210)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-PLK1 (T210) antibody (Cat#1690). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PLK1 (T210) antibody (Cat#1690, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Phospho-PLK1 (T210) antibody (Cat#1690, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PLK1 (T210) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PLK1
  • Catalog：
    1690
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using Phospho-PLK1 (T210) antibody (Cat#1690). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PLK1 (T210) antibody (Cat#1690, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Phospho-PLK1 (T210) antibody (Cat#1690, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PLK1 (T210) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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