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Home>Product>Primary Antibody

Histone H2A (Hydroxyl Y39) Recombinant Rabbit mAb

#2118

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Recombinant Antibody
Western blotting analysis using Histone H2A (hydroxyl Y39) antibody (Cat#2118). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H2A (hydroxyl Y39) antibody (Cat#2118, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Histone H2A (hydroxyl Y39) antibody (Cat#2118, 1:1,000). Nuclei were stained blue with DAPI; Histone H2A (hydroxyl Y39) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Histone H2A (hydroxyl Y39) expression in HepG2 cells using Histone H2A (hydroxyl Y39) antibody (Cat#2118, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Histone H2A (hydroxyl Y39).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H2AC4
  • Catalog：
    2118
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Histone H2A (Hydroxyl Y39) Recombinant Rabbit mAb
Aliases H2AC4; H2A Clustered Histone 4; HIST1H2AB; H2A/M; H2AFM; Histone Cluster 1 H2A Family Member B; H2A Histone Family, Member M; Histone Cluster 1, H2ab; Histone H2A Type 1-B/E; Histone 1, H2ab; Histone H2A/M; Histone H2A.2; Histone H2A/A; HIST1H2AE; H2AC8; H2AFA
Background

Gene Name: H2AC4

NCBI Gene Entry: 8335

UniProt Entry: P04908

Application Information

Molecular Weight: Predicted, 14 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6085

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Histone H2A (hydroxyl Y39)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Histone H2A (hydroxyl Y39) antibody (Cat#2118). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H2A (hydroxyl Y39) antibody (Cat#2118, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Histone H2A (hydroxyl Y39) antibody (Cat#2118, 1:1,000). Nuclei were stained blue with DAPI; Histone H2A (hydroxyl Y39) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Histone H2A (hydroxyl Y39) expression in HepG2 cells using Histone H2A (hydroxyl Y39) antibody (Cat#2118, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Histone H2A (hydroxyl Y39).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H2AC4
  • Catalog：
    2118
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Histone H2A (hydroxyl Y39) antibody (Cat#2118). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H2A (hydroxyl Y39) antibody (Cat#2118, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Histone H2A (hydroxyl Y39) antibody (Cat#2118, 1:1,000). Nuclei were stained blue with DAPI; Histone H2A (hydroxyl Y39) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Histone H2A (hydroxyl Y39) expression in HepG2 cells using Histone H2A (hydroxyl Y39) antibody (Cat#2118, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Histone H2A (hydroxyl Y39).
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