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Home>Product>Primary Antibody

Laminin Subunit Gamma 1 Recombinant Rabbit mAb

#2187

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Recombinant Antibody
Western blotting analysis using Laminin subunit gamma 1 antibody (Cat#2187). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Laminin subunit gamma 1 antibody (Cat#2187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Laminin subunit gamma 1 antibody (Cat#2187, 1:1,000). Nuclei were stained blue with DAPI; Laminin subunit gamma 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Laminin subunit gamma 1 expression in HAP-1 cells using Laminin subunit gamma 1 antibody (Cat#2187, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Laminin subunit gamma 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LAMC1
  • Catalog：
    2187
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name Laminin Subunit Gamma 1 Recombinant Rabbit mAb
Aliases LAMC1; Laminin Subunit Gamma 1; Laminin B2 Chain; LAMB2; Laminin, Gamma 1 (Formerly LAMB2); Laminin-10 Subunit Gamma; Laminin-11 Subunit Gamma; Laminin Subunit Gamma-1; S-Laminin Subunit Gamma; Laminin-2 Subunit Gamma; Laminin-3 Subunit Gamma; Laminin-4 Subunit Gamma; Laminin-6 Subunit Gamma; Laminin-7 Subunit Gamma; Laminin-8 Subunit Gamma; Laminin-9 Subunit Gamma; S-LAM Gamma; Laminin-1 Subunit Gamma
Background

Gene Name: LAMC1

NCBI Gene Entry: 3915

UniProt Entry: P11047

Application Information

Molecular Weight: Predicted, 178 kDa; observed, 220-250 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6415

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Laminin gamma 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Laminin subunit gamma 1 antibody (Cat#2187). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Laminin subunit gamma 1 antibody (Cat#2187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with Laminin subunit gamma 1 antibody (Cat#2187, 1:1,000). Nuclei were stained blue with DAPI; Laminin subunit gamma 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Laminin subunit gamma 1 expression in HAP-1 cells using Laminin subunit gamma 1 antibody (Cat#2187, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Laminin subunit gamma 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LAMC1
  • Catalog：
    2187
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using Laminin subunit gamma 1 antibody (Cat#2187). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Laminin subunit gamma 1 antibody (Cat#2187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Laminin subunit gamma 1 antibody (Cat#2187, 1:1,000). Nuclei were stained blue with DAPI; Laminin subunit gamma 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Laminin subunit gamma 1 expression in HAP-1 cells using Laminin subunit gamma 1 antibody (Cat#2187, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Laminin subunit gamma 1.
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