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Home>Product>Reagents

FeQ™ ECL Substrate Kit

#226

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HEK293 cell lysates were separated by SDS-PAGE and immunoblotted with anti- ACTB (β-actin) antibody. The micrograms of total cell lysates were indicated on top of the graph. Letter A, a widely used ECL from a commercial vendor; B, PiQ™ECL; and C, FeQ™ ECL.The exposure time was indicated on the right.
30 μg of lysates from HeLa (human,A)and DF-1(chicken, B) cells were separated by SDS-PAGE and immunoblotted using anti-RRM1antibody (90 kDa). Comparison of PiQ™ and FeQ™ ECLs to an ECL from vendor X showed that our products outperformed the brand of vendor X. In particular, when it comes to weak band, our ECL products were much more sensitive and powerful than Brand-X.
A total of 30 μg of cell lysates from various cell lines were separated by SDS-PAGE and immunoblotted using anti-DLAT antibody (69 kDa). The same blot was first exposed for 30 seconds (A) using an ECL reagent from vendor X for 30 seconds, washed thoroughly with TBST, then exposed for 5 seconds using our FeQ™ECL(B). Note that the existing bands in HeLa cells were greatly enhanced, and the band not detected by the vendor's ECL appeared in the pig cell line using FeQ™ ECL.
Please select the specifications of the product.
Specification：
50 mL 100 mL 200 mL
Unit price：
$119.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    -
  • Catalog：
    226
  • Application：
    WB
  • Species Reactivity：
    -

Information

Data

Information

Background

Enhanced chemiluminescence (ECL) reagents are used with horseradish peroxidase (HRP)-conjugated secondary antibodies to detect protein abundance in Western blot. HRP catalyzes luminol oxidation in the presence of hydrogen peroxide, producing light that can be detected by X-ray film and digital imagers.

FeQ™ ECL Substrate Kit is formulated with proprietary enhancers that greatly increase the intensity and duration of emitted light. FeQ™ ECL reagents are ultrasensitive and suitable to detect proteins at femtogram level. When using these reagents, the recommended dilutions for primary and secondary antibodies are 1:10,000 and 1:100,000, respectively. Compared to other commonly used ECL reagents, ours exhibit superior performance by generating extremely intense signal with low background and require ten-fold less primary and secondary antibodies.

Storage

Stable at 4°C for at least 1 year

Experimental Protocol

  1. Allow the ECL reagents to equilibrate to room temperature approximately 30 min before use.
  2. Wash the membrane thoroughly to reduce non-specific signals.Mix Reagent A and B at a 1:1 ratio. Vortex to mix.
  3. Immerse the membrane in the mixed solution for 2–3 min.
  4. Drain the excess solution using a paper towel and wrap the membrane in plastic wrap or a clear plastic folder.
  5. Detect the chemiluminescence using X-ray film or a digital imager.
Data
  • HEK293 cell lysates were separated by SDS-PAGE and immunoblotted with anti- ACTB (β-actin) antibody. The micrograms of total cell lysates were indicated on top of the graph. Letter A, a widely used ECL from a commercial vendor; B, PiQ™ECL; and C, FeQ™ ECL.The exposure time was indicated on the right.
  • 30 μg of lysates from HeLa (human,A)and DF-1(chicken, B) cells were separated by SDS-PAGE and immunoblotted using anti-RRM1antibody (90 kDa). Comparison of PiQ™ and FeQ™ ECLs to an ECL from vendor X showed that our products outperformed the brand of vendor X. In particular, when it comes to weak band, our ECL products were much more sensitive and powerful than Brand-X.
  • A total of 30 μg of cell lysates from various cell lines were separated by SDS-PAGE and immunoblotted using anti-DLAT antibody (69 kDa). The same blot was first exposed for 30 seconds (A) using an ECL reagent from vendor X for 30 seconds, washed thoroughly with TBST, then exposed for 5 seconds using our FeQ™ECL(B). Note that the existing bands in HeLa cells were greatly enhanced, and the band not detected by the vendor's ECL appeared in the pig cell line using FeQ™ ECL.
Please select the specifications of the product.
Specification：
50 mL 100 mL 200 mL
Unit price：
$119.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    -
  • Catalog：
    226
  • Application：
    WB
  • Species Reactivity：
    -
RELATED PRODUCTS
HEK293 cell lysates were separated by SDS-PAGE and immunoblotted with anti- ACTB (β-actin) antibody. The micrograms of total cell lysates were indicated on top of the graph. Letter A, a widely used ECL from a commercial vendor; B, PiQ™ECL; and C, FeQ™ ECL.The exposure time was indicated on the right.
30 μg of lysates from HeLa (human,A)and DF-1(chicken, B) cells were separated by SDS-PAGE and immunoblotted using anti-RRM1antibody (90 kDa). Comparison of PiQ™ and FeQ™ ECLs to an ECL from vendor X showed that our products outperformed the brand of vendor X. In particular, when it comes to weak band, our ECL products were much more sensitive and powerful than Brand-X.
A total of 30 μg of cell lysates from various cell lines were separated by SDS-PAGE and immunoblotted using anti-DLAT antibody (69 kDa). The same blot was first exposed for 30 seconds (A) using an ECL reagent from vendor X for 30 seconds, washed thoroughly with TBST, then exposed for 5 seconds using our FeQ™ECL(B). Note that the existing bands in HeLa cells were greatly enhanced, and the band not detected by the vendor's ECL appeared in the pig cell line using FeQ™ ECL.
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