Background

Enhanced chemiluminescence (ECL) reagents are used with horseradish peroxidase (HRP)-conjugated secondary antibodies to detect protein abundance in Western blot. HRP catalyzes luminol oxidation in the presence of hydrogen peroxide, producing light that can be detected by X-ray film and digital imagers. Our NaQ™ reagents are formulated with proprietary enhancers that greatly increase the intensity and duration of emitted light.

Our NaQ™ ECL reagents are suitable to detect proteins within the high picogram to low nanogram range. When using NaQ™ ECL reagents, the recommended dilutions for primary and secondary antibodies are 1:1,000-2,000 and 1:10,000-20,000, respectively. Compared to other commonly used ECL reagents, ours exhibit excellent performance by generating intense signal with low background and require less primary and secondary antibodies.

Storage

Stable at 4°C for at least 1 year

Experimental Protocol

1. Allow the ECL reagents to equilibrate to room temperature approximately 30 min before use.
2. Wash the membrane thoroughly to reduce non-specific signals.
3. Mix Reagent A and B at a 1:1 ratio. Vortex to mix.
4. Immerse the membrane in the mixed solution for 2–3 min.
5. Drain the excess solution using a paper towel and wrap the membrane in plastic wrap or a clear plastic folder.
6. Detect the chemiluminescence using X-ray film or a digital imager.