KLF Transcription Factor 4 Mouse mAb

#2832

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Western blotting analysis using KLF4 antibody (Cat#2832). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KLF4 antibody (Cat#2832, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Immunocytochemical staining of Hela cells with KLF4 antibody (Cat#2832, 1:1,000). Nuclei were stained blue with DAPI; KLF4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.

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Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

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(Excluding sales tax)

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Gene Symbol：

KLF4
Catalog：

2832
Application：

WB, ICC
Species Reactivity：

H

Information

Data

Information

Product Name	KLF Transcription Factor 4 Mouse mAb
Aliases	KLF4; KLF Transcription Factor 4; GKLF; EZF; Epithelial Zinc Finger Protein EZF; Gut-Enriched Krueppel-Like Factor; Kruppel-Like Factor 4 (Gut); Gut Kruppel-Like Factor; Krueppel-Like Factor 4; Kruppel Like Factor 4; Endothelial Kruppel-Like Zinc Finger Protein
Background	Gene Name: KLF4 NCBI Gene Entry: 9314 UniProt Entry: O43474
Application Information	Molecular Weight: Predicted, 55 kDa; observed, 70 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB2750 Species Reactivity: Human Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen	Recombinant protein of human KLF4
Isotype	Mouse IgG2b kappa
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using KLF4 antibody (Cat#2832). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KLF4 antibody (Cat#2832, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with KLF4 antibody (Cat#2832, 1:1,000). Nuclei were stained blue with DAPI; KLF4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

KLF4
Catalog：

2832
Application：

WB, ICC
Species Reactivity：

H

Datasheet

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