Western blotting analysis using DCAF13 antibody (Cat#3036). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCAF13 antibody (Cat#3036, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with DCAF13 antibody (Cat#3036, 1:1,000). Nuclei were stained blue with DAPI; DCAF13 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DCAF13 expression in HepG2 cells using DCAF13 antibody (Cat#3036, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DCAF13.
DDB1 And CUL4 Associated Factor 1 Recombinant Rabbit mAb
Aliases
DCAF13; DDB1 And CUL4 Associated Factor 1; HSPC064; WDSOF1; Gm83; Sof; WD Repeat And SOF Domain-Containing Protein 1; WD Repeats And SOF1 Domain Containing; DDB1- And CUL4-Associated Factor 13; DKFZP564O0463
Western blotting analysis using DCAF13 antibody (Cat#3036). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCAF13 antibody (Cat#3036, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with DCAF13 antibody (Cat#3036, 1:1,000). Nuclei were stained blue with DAPI; DCAF13 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DCAF13 expression in HepG2 cells using DCAF13 antibody (Cat#3036, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DCAF13.
Western blotting analysis using DCAF13 antibody (Cat#3036). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCAF13 antibody (Cat#3036, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with DCAF13 antibody (Cat#3036, 1:1,000). Nuclei were stained blue with DAPI; DCAF13 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DCAF13 expression in HepG2 cells using DCAF13 antibody (Cat#3036, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DCAF13.
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