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Home>Product>Primary Antibody

Fc Gamma Receptor IIIa Recombinant Rabbit mAb

#3048

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Recombinant Antibody
Western blotting analysis using CD16 antibody (Cat#3048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD16 antibody (Cat#3048, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with CD16 antibody(Cat#3048, 1:1,000). Nuclei were stained blue with DAPI; CD16 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD16 expression in HT-1080 cells using CD16 antibody (Cat#3048, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD16.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FCGR3A
  • Catalog：
    3048
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Fc Gamma Receptor IIIa Recombinant Rabbit mAb
Aliases FCGR3A; Fc Gamma Receptor IIIa; FcgammaRIIIa; FcGRIIIA; CD16a; FCGR3; CD16; FCG3; Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; Fc Fragment Of IgG, Low Affinity IIIa, Receptor (CD16a); Fc Fragment Of IgG Receptor IIIa; IgG Fc Receptor III-A; IgG Fc Receptor III-2; Fc-Gamma RIII-Alpha; CD16a Antigen; CD16-II; IGFR3; Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A/III-B Recombinant; Low Affinity Immunoglobulin Gamma Receptor III-A Fc Fragment; Fc Fragment Of IgG, Low Affinity IIIa, Receptor For (CD16); Low Affinity Immunoglobulin Gamma Fc Region Receptor III-B; Fc Fragment Of IgG, Low Affinity III, Receptor For (CD16); Immunoglobulin G Fc Receptor III; Fc-Gamma Receptor III-2 (CD 16); Neutrophil-Specific Antigen NA; Fc-Gamma Receptor IIIb (CD16); Fc Gamma Receptor III-A; Fc-Gamma RIIIa; Fc-Gamma RIII; FcgammaRIIIA; FCGRIII; FCRIIIA; FcRIIIa; FCR-10; FCRIII; FcRIII; FcR-10; IMD20; CD16A
Background

Gene Name: FCGR3A

NCBI Gene Entry: 2214

UniProt Entry: P08637

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3805

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CD16
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD16 antibody (Cat#3048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD16 antibody (Cat#3048, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with CD16 antibody(Cat#3048, 1:1,000). Nuclei were stained blue with DAPI; CD16 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CD16 expression in HT-1080 cells using CD16 antibody (Cat#3048, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD16.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FCGR3A
  • Catalog：
    3048
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CD16 antibody (Cat#3048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD16 antibody (Cat#3048, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with CD16 antibody(Cat#3048, 1:1,000). Nuclei were stained blue with DAPI; CD16 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD16 expression in HT-1080 cells using CD16 antibody (Cat#3048, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD16.
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