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SLAMF6 Recombinant Rabbit mAb

#3689

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Recombinant Antibody
Western blotting analysis using SLAMF6 antibody (Cat#3689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SLAMF6 antibody (Cat#3689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Raji cells with SLAMF6 antibody (Cat#3689, 1:1,000). Nuclei were stained blue with DAPI; SLAMF6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SLAMF6 expression in Raji cells using SLAMF6 antibody (Cat#3689, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SLAMF6.
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Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    SLAMF6
  • Catalog:
    3689
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name SLAMF6 Recombinant Rabbit mAb
Aliases SLAMF6; SLAM Family Member 6; NTB-A; KALI; SF2000; KALIb; Ly108; CD352; NTBA; Activating NK Receptor; NK-T-B-Antigen; Natural Killer-, T- And B-Cell Antigen; NTBA Receptor; CD352 Antigen
Background

Gene Name: SLAMF6

NCBI Gene Entry: 114836

UniProt Entry: Q96DU3

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 60 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6920

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human SLAMF6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SLAMF6 antibody (Cat#3689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SLAMF6 antibody (Cat#3689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Raji cells with SLAMF6 antibody (Cat#3689, 1:1,000). Nuclei were stained blue with DAPI; SLAMF6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SLAMF6 expression in Raji cells using SLAMF6 antibody (Cat#3689, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SLAMF6.
Western blotting analysis using SLAMF6 antibody (Cat#3689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SLAMF6 antibody (Cat#3689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Raji cells with SLAMF6 antibody (Cat#3689, 1:1,000). Nuclei were stained blue with DAPI; SLAMF6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SLAMF6 expression in Raji cells using SLAMF6 antibody (Cat#3689, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SLAMF6.
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