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Home>Product>Primary Antibody

Atg4C Recombinant Rabbit mAb

#4336

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Recombinant Antibody
Western blotting analysis using ATG4C antibody (Cat#4336). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG4C antibody (Cat#4336, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Atg4C antibody (Cat#4336, 1:1,000). Nuclei were stained blue with DAPI; Atg4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Atg4C expression in HepG2 cells using Atg4C antibody (Cat#4336, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Atg4C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATG4C
  • Catalog：
    4336
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Atg4C Recombinant Rabbit mAb
Aliases ATG4C; Autophagy Related 4C Cysteine Peptidase; AUTL3; APG4C; AUTL1; Autophagy-Related Cysteine Endopeptidase 3; Autophagy-Related Protein 4 Homolog C; AUT-Like 3 Cysteine Endopeptidase; Cysteine Protease ATG4C; Autophagin-3; FLJ14867; HsAPG4C; AUT (S. Cerevisiae)-Like 1, Cysteine Endopeptidase; AUT-Like 1, Cysteine Endopeptidase (S. Cerevisiae); ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae); APG4 Autophagy 4 Homolog C (S. Cerevisiae); Epididymis Secretory Sperm Binding Protein; Autophagy Related 4C, Cysteine Peptidase; ATG4 Autophagy Related 4 Homolog C; AUT-Like 1, Cysteine Endopeptidase; APG4 Autophagy 4 Homolog C; EC 3.4.22.-; EC 3.4.22; APG4-C
Background

Gene Name: ATG4C

NCBI Gene Entry: 84938

UniProt Entry: Q96DT6

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10710

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ATG4C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATG4C antibody (Cat#4336). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG4C antibody (Cat#4336, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Atg4C antibody (Cat#4336, 1:1,000). Nuclei were stained blue with DAPI; Atg4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Atg4C expression in HepG2 cells using Atg4C antibody (Cat#4336, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Atg4C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATG4C
  • Catalog：
    4336
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using ATG4C antibody (Cat#4336). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG4C antibody (Cat#4336, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Atg4C antibody (Cat#4336, 1:1,000). Nuclei were stained blue with DAPI; Atg4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Atg4C expression in HepG2 cells using Atg4C antibody (Cat#4336, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Atg4C.
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