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HAUS7 Recombinant Rabbit mAb

#4434

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Recombinant Antibody
Western blotting analysis using HAUS7 antibody (Cat#4434). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HAUS7 antibody (Cat#4434, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with HAUS7 antibody (Cat#4434, 1:1,000). Nuclei were stained blue with DAPI; HAUS7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HAUS7 expression in HepG2 cells using HAUS7 antibody (Cat#4434, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HAUS7.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    HAUS7
  • Catalog:
    4434
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name HAUS7 Recombinant Rabbit mAb
Aliases HAUS7; HAUS Augmin Like Complex Subunit 7; UIP1; UCHL5IP; 26S Proteasome-Associated UCH37-Interacting Protein 1; HAUS Augmin-Like Complex Subunit 7; X-Linked Protein STS1769; 26S Proteasome-Associated UCH Interacting Protein 1; UCH37 Interacting Protein 1; UCHL5 Interacting Protein; UCHL5-Interacting Protein
Background

Gene Name: HAUS7

NCBI Gene Entry: 55559

UniProt Entry: Q99871

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11200

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human UCHL5IP
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HAUS7 antibody (Cat#4434). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HAUS7 antibody (Cat#4434, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with HAUS7 antibody (Cat#4434, 1:1,000). Nuclei were stained blue with DAPI; HAUS7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of HAUS7 expression in HepG2 cells using HAUS7 antibody (Cat#4434, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HAUS7.
Western blotting analysis using HAUS7 antibody (Cat#4434). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HAUS7 antibody (Cat#4434, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with HAUS7 antibody (Cat#4434, 1:1,000). Nuclei were stained blue with DAPI; HAUS7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HAUS7 expression in HepG2 cells using HAUS7 antibody (Cat#4434, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HAUS7.
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