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Home>Product>Primary Antibody

RASGRP1 Recombinant Rabbit mAb

#5636

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Recombinant Antibody
Western blotting analysis using RASGRP1 antibody (Cat#5636). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RASGRP1 antibody (Cat#5636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of RASGRP1 expression in Jurkat cells using RASGRP1 antibody (Cat#5636, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RASGRP1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RASGRP1
  • Catalog：
    5636
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name RASGRP1 Recombinant Rabbit mAb
Aliases RASGRP1; RAS Guanyl Releasing Protein 1; CalDAG-GEFII; RASGRP; Calcium- And Diacylglycerol-Regulated Guanine Nucleotide Exchange Factor II; Calcium And DAG-Regulated Guanine Nucleotide Exchange Factor II; RAS Guanyl Releasing Protein 1 (Calcium And DAG-Regulated); RAS Guanyl-Releasing Protein 1; Guanine Nucleotide Exchange Factor, Calcium- And DAG-Regulated, Rap1A; RAS Guanyl Nucleotide-Releasing Protein 1; Ras Guanyl-Releasing Protein; Ras Activator RasGRP; CALDAG-GEFI; IMD64
Background

Gene Name: RASGRP1

NCBI Gene Entry: 10125

UniProt Entry: O95267

Application Information

Molecular Weight: Predicted, 90 kDa; observed, 90 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4630

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human RASGRP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using RASGRP1 antibody (Cat#5636). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RASGRP1 antibody (Cat#5636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of RASGRP1 expression in Jurkat cells using RASGRP1 antibody (Cat#5636, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RASGRP1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RASGRP1
  • Catalog：
    5636
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using RASGRP1 antibody (Cat#5636). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RASGRP1 antibody (Cat#5636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of RASGRP1 expression in Jurkat cells using RASGRP1 antibody (Cat#5636, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RASGRP1.
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