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TEAD1 Recombinant Rabbit mAb

#4661

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Recombinant Antibody
Western blotting analysis using TEAD1 antibody (Cat#4661). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TEAD1 antibody (Cat#4661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with TEAD1 antibody (Cat#4661, 1:1,000). Nuclei were stained blue with DAPI; TEAD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    TEAD1
  • Catalog:
    4661
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name TEAD1 Recombinant Rabbit mAb
Aliases TEAD1; TEA Domain Transcription Factor 1; TEF-1; TCF13; TEA Domain Family Member 1 (SV40 Transcriptional Enhancer Factor); Transcriptional Enhancer Factor TEF-1; Transcriptional Enhancer Factor 1; Transcription Factor 13; Protein GT-IIC; NTEF-1; TCF-13; TEAD-1; AA; Atrophia Areata, Peripapillary Chorioretinal Degeneration; TEA Domain Family Member 1; REF1; TEF1
Background

Gene Name: TEAD1

NCBI Gene Entry: 7003

UniProt Entry: P28347

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12315

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human TEAD1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TEAD1 antibody (Cat#4661). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TEAD1 antibody (Cat#4661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with TEAD1 antibody (Cat#4661, 1:1,000). Nuclei were stained blue with DAPI; TEAD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using TEAD1 antibody (Cat#4661). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TEAD1 antibody (Cat#4661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with TEAD1 antibody (Cat#4661, 1:1,000). Nuclei were stained blue with DAPI; TEAD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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