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KD-Validated FAM50A Recombinant Rabbit mAb

#61984

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using FAM50A antibody (Cat#61984). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FAM50A antibody (Cat#61984, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FAM50A antibody (Cat#61984). FAM50A expression in wild-type (WT) and FAM50A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FAM50A antibody (Cat#61984, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    FAM50A
  • Catalog:
    61984
  • Application:
    WB
  • Species Reactivity:
    H, M, R
Information
Product Name KD-Validated FAM50A Recombinant Rabbit mAb
Aliases FAM50A; Family With Sequence Similarity 50 Member A; DXS9928E; XAP5; HXC-26; 9F; Protein FAM50A; Protein XAP-; HXC26; DNA Segment On Chromosome X (Unique) 9928 Expressed Sequence; Armfield X-Linked Mental Retardation Syndrome; Family With Sequence Similarity 50, Member A; Protein HXC-26; MRXSA
Background

Gene Name: FAM50A

NCBI Gene Entry: 9130

UniProt Entry: Q14320

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6825

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human FAM50A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using FAM50A antibody (Cat#61984). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FAM50A antibody (Cat#61984, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using FAM50A antibody (Cat#61984). FAM50A expression in wild-type (WT) and FAM50A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FAM50A antibody (Cat#61984, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FAM50A antibody (Cat#61984). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FAM50A antibody (Cat#61984, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FAM50A antibody (Cat#61984). FAM50A expression in wild-type (WT) and FAM50A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FAM50A antibody (Cat#61984, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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