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Home>Product>Primary Antibody

Tumor Protein P63 Mouse mAb

#4877

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Western blotting analysis using TP63 antibody (Cat#4877). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TP63 antibody (Cat#4877, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HaCaT cells with TP63 antibody (Cat#4877, 1:1,000). Nuclei were stained blue with DAPI; TP63 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TP63 expression in HaCaT cells using TP63 antibody (Cat#4877, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TP63.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TP63
  • Catalog：
    4877
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Tumor Protein P63 Mouse mAb
Aliases TP63; Tumor Protein P63; P73L; P63; P73H; P51; KET; P40; SHFM4; P53CP; TP73L; EEC3; OFC8; NBP; Chronic Ulcerative Stomatitis Protein; Keratinocyte Transcription Factor KET; Tumor Protein P53-Competing Protein; Transformation-Related Protein 63; Tumor Protein P73-Like; Tumor Protein 63; TP53CP; TP53L; Amplified In Squamous Cell Carcinoma; Tumor Protein P53-Like; B(P51A); B(P51B); CUSP; AIS; LMS; RHS
Background

Gene Name: TP63

NCBI Gene Entry: 8626

UniProt Entry: Q9H3D4

Application Information

Molecular Weight: Predicted, 77 kDa; observed, 77 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13390

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human p40
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TP63 antibody (Cat#4877). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TP63 antibody (Cat#4877, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HaCaT cells with TP63 antibody (Cat#4877, 1:1,000). Nuclei were stained blue with DAPI; TP63 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of TP63 expression in HaCaT cells using TP63 antibody (Cat#4877, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TP63.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TP63
  • Catalog：
    4877
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using TP63 antibody (Cat#4877). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TP63 antibody (Cat#4877, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HaCaT cells with TP63 antibody (Cat#4877, 1:1,000). Nuclei were stained blue with DAPI; TP63 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TP63 expression in HaCaT cells using TP63 antibody (Cat#4877, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TP63.
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