Western blotting analysis using peptidylprolyl isomerase C antibody (Cat#5237). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with peptidylprolyl isomerase C antibody (Cat#5237, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Peptidylprolyl isomerase C expression in HepG2 cells using Peptidylprolyl isomerase C antibody (Cat#5237, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Peptidylprolyl isomerase C.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human PPIC
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using peptidylprolyl isomerase C antibody (Cat#5237). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with peptidylprolyl isomerase C antibody (Cat#5237, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Peptidylprolyl isomerase C expression in HepG2 cells using Peptidylprolyl isomerase C antibody (Cat#5237, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Peptidylprolyl isomerase C.
Western blotting analysis using peptidylprolyl isomerase C antibody (Cat#5237). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with peptidylprolyl isomerase C antibody (Cat#5237, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Peptidylprolyl isomerase C expression in HepG2 cells using Peptidylprolyl isomerase C antibody (Cat#5237, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Peptidylprolyl isomerase C.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
