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Home>Product>Primary Antibody

NONO Recombinant Rabbit mAb

#5536

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Recombinant Antibody
Western blotting analysis using NONO antibody (Cat#5536). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NONO antibody (Cat#5536, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with NONO antibody (Cat#5536, 1:1,000). Nuclei were stained blue with DAPI; NONO was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NONO expression in HepG2 cells using NONO antibody (Cat#5536, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NONO.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NONO
  • Catalog：
    5536
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name NONO Recombinant Rabbit mAb
Aliases NONO; Non-POU Domain Containing Octamer Binding; NRB54; NMT55; PPP1R114; P54NRB; P54; Non-Pou Domain-Containing Octamer (ATGCAAAT) Binding Protein; Non-POU Domain-Containing Octamer-Binding Protein; Protein Phosphatase 1, Regulatory Subunit 114; DNA-Binding P52/P100 Complex, 52 KDa Subunit; 54 KDa Nuclear RNA- And DNA-Binding Protein; 55 KDa Nuclear Protein; P54(Nrb); Non-POU-Domain-Containing, Octamer-Binding; Nuclear RNA-Binding Protein, 54-KD; NonO Protein; MRXS34; P54nrb
Background

Gene Name: NONO

NCBI Gene Entry: 4841

UniProt Entry: Q15233

Application Information

Molecular Weight: Predicted, 54 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1960

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human NONO
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NONO antibody (Cat#5536). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NONO antibody (Cat#5536, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with NONO antibody (Cat#5536, 1:1,000). Nuclei were stained blue with DAPI; NONO was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of NONO expression in HepG2 cells using NONO antibody (Cat#5536, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NONO.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NONO
  • Catalog：
    5536
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using NONO antibody (Cat#5536). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NONO antibody (Cat#5536, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with NONO antibody (Cat#5536, 1:1,000). Nuclei were stained blue with DAPI; NONO was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NONO expression in HepG2 cells using NONO antibody (Cat#5536, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NONO.
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