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Home>Product>Primary Antibody

RAD18 Recombinant Rabbit mAb

#2446

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Recombinant Antibody
Western blotting analysis using RAD18 antibody (Cat#2446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD18 antibody (Cat#2446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9c2 cells with RAD18 antibody (Cat#2446, 1:1,000). Nuclei were stained blue with DAPI; RAD18 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RAD18 expression in H9c2 cells using RAD18 antibody (Cat#2446, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RAD18.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RAD18
  • Catalog：
    2446
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name RAD18 Recombinant Rabbit mAb
Aliases RAD18 E3 Ubiquitin Protein Ligase; RNF73; RING-Type E3 Ubiquitin Transferase RAD18; Postreplication Repair Protein RAD18; E3 Ubiquitin-Protein Ligase RAD18; RING Finger Protein 73; HRAD18; HHR18; Postreplication Repair Protein HRAD18p; RAD18 Homolog (S. Cerevisiae); RAD18, S. Cerevisiae, Homolog; RAD18 Homolog; EC 2.3.2.27
Background

Gene Name: RAD18

NCBI Gene Entry: 56852

UniProt Entry: Q9NS91

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB880

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human RAD18
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RAD18 antibody (Cat#2446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD18 antibody (Cat#2446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of H9c2 cells with RAD18 antibody (Cat#2446, 1:1,000). Nuclei were stained blue with DAPI; RAD18 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of RAD18 expression in H9c2 cells using RAD18 antibody (Cat#2446, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RAD18.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RAD18
  • Catalog：
    2446
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using RAD18 antibody (Cat#2446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD18 antibody (Cat#2446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9c2 cells with RAD18 antibody (Cat#2446, 1:1,000). Nuclei were stained blue with DAPI; RAD18 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RAD18 expression in H9c2 cells using RAD18 antibody (Cat#2446, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RAD18.
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