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Home>Product>Primary Antibody

HTR2C Recombinant Rabbit mAb

#5549

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Recombinant Antibody
Western blotting analysis using HTR2C antibody (Cat#5549). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HTR2C antibody (Cat#5549, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with HTR2C antibody (Cat#5549, 1:1,000). Nuclei were stained blue with DAPI; HTR2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HTR2C expression in HepG2 cells using HTR2C antibody (Cat#5549, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HTR2C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HTR2C
  • Catalog：
    5549
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name HTR2C Recombinant Rabbit mAb
Aliases HTR2C; 5-Hydroxytryptamine Receptor 2C; 5-HT2C; 5HTR2C; HTR1C; 5-Hydroxytryptamine (Serotonin) Receptor 2C, G Protein-Coupled; 5-Hydroxytryptamine Receptor 1C; 5-HTR2C; 5-HT1C; 5-Hydroxytryptamine (Serotonin) Receptor 2C; Serotonin 5-HT-1C Receptor; Serotonin 5-HT-2C Receptor; Serotonin Receptor 2C; 5-HT-2C; 5-HT-1C
Background

Gene Name: HTR2C

NCBI Gene Entry: 3358

UniProt Entry: P28335

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 65 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2025

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human 5 HT2C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HTR2C antibody (Cat#5549). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HTR2C antibody (Cat#5549, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with HTR2C antibody (Cat#5549, 1:1,000). Nuclei were stained blue with DAPI; HTR2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of HTR2C expression in HepG2 cells using HTR2C antibody (Cat#5549, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HTR2C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HTR2C
  • Catalog：
    5549
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using HTR2C antibody (Cat#5549). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HTR2C antibody (Cat#5549, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with HTR2C antibody (Cat#5549, 1:1,000). Nuclei were stained blue with DAPI; HTR2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HTR2C expression in HepG2 cells using HTR2C antibody (Cat#5549, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HTR2C.
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