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PHLDA1 Recombinant Rabbit mAb

#5589

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Recombinant Antibody
Western blotting analysis using PHLDA1 antibody (Cat#5589). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PHLDA1 antibody (Cat#5589, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PHLDA1 expression in HT-1080 cells using PHLDA1 antibody (Cat#5589, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PHLDA1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PHLDA1
  • Catalog:
    5589
  • Application:
    WB, FCM
  • Species Reactivity:
    H, R
Information
Product Name PHLDA1 Recombinant Rabbit mAb
Aliases PHLDA1; Pleckstrin Homology Like Domain Family A Member 1; TDAG51; PHRIP; DT1P1B11; Pleckstrin Homology-Like Domain Family A Member 1; T-Cell Death-Associated Gene 51 Protein; Apoptosis-Associated Nuclear Protein; Proline- And Glutamine-Rich Protein; Proline- And Histidine-Rich Protein; Proline-Histidine Rich Protein; PQ-Rich Protein; PQR Protein; Pleckstrin Homology-Like Domain, Family A, Member 1; T-Cell Death-Associated Gene 51
Background

Gene Name: PHLDA1

NCBI Gene Entry: 22822

UniProt Entry: Q8WV24

Application Information

Molecular Weight: Predicted, 45 kDa; observed, 38 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4405

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PHLDA1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PHLDA1 antibody (Cat#5589). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PHLDA1 antibody (Cat#5589, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of PHLDA1 expression in HT-1080 cells using PHLDA1 antibody (Cat#5589, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PHLDA1.
Western blotting analysis using PHLDA1 antibody (Cat#5589). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PHLDA1 antibody (Cat#5589, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PHLDA1 expression in HT-1080 cells using PHLDA1 antibody (Cat#5589, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PHLDA1.
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