KD-Validated RAB5C Recombinant Rabbit mAb - Product

KD-Validated RAB5C Recombinant Rabbit mAb

#61660

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Recombinant Antibody
Knockdown Validated

Western blotting analysis using RAB5C antibody (Cat#61660). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAB5C antibody (Cat#61660, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using RAB5C antibody (Cat#61660). RAB5C expression in wild type (WT) and RAB5C knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAB5C antibody (Cat#61660, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of RAB5C expression in HepG2 cells using RAB5C antibody (Cat#61660, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RAB5C.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

RAB5C
Catalog：

61660
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated RAB5C Recombinant Rabbit mAb
Aliases	RAB5C; RAB5C, Member RAS Oncogene Family; RAB5CL; RABL; RAB5C, Member Of RAS Oncogene Family; Ras-Related Protein Rab-5C; L1880; RAB5L; RAB, Member Of RAS Oncogene Family-Like; EC 3.6.5.2
Background	Gene Name: RAB5C NCBI Gene Entry: 5878 UniProt Entry: P51148
Application Information	Molecular Weight: Predicted, 23 kDa; observed, 23 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB3190 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human RAB5C / RABL
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using RAB5C antibody (Cat#61660). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAB5C antibody (Cat#61660, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RAB5C antibody (Cat#61660). RAB5C expression in wild type (WT) and RAB5C knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAB5C antibody (Cat#61660, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of RAB5C expression in HepG2 cells using RAB5C antibody (Cat#61660, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RAB5C.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

RAB5C
Catalog：

61660
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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