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Home>Product>Primary Antibody

KD-Validated GPRC5A Recombinant Rabbit mAb

#67017

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using GPRC5A antibody (Cat#67017, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using GPRC5A antibody (Cat#67017, 1:1,000). GPRC5A expression in wild-type (WT) and GPRC5A shRNA knockdown (KD) MCF7 cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using GPRC5A antibody (Cat#67017). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GPRC5A antibody (Cat#67017, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively.
Immunocytochemical staining of MCF7 cells using GPRC5A antibody (Cat#67017, 1:100). Top panel: wild type (WT); Bottom panel: GPRC5A shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; GPRC5A was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Flow cytometric analysis of GPRC5A expression in HeLa cells using GPRC5A antibody (Cat#67017, 1:100). Red, isotype control; blue, GPRC5A.
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Unit price：
Quantity：
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  • Gene Symbol：
    GPRC5A
  • Catalog：
    67017
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated GPRC5A Recombinant Rabbit mAb
Aliases GPRC5A; G Protein-Coupled Receptor Class C Group 5 Member A; PEIG-1; RAIG1; TIG1; RAI3; G-Protein Coupled Receptor Family C Group 5 Member A; Retinoic Acid-Induced Gene 1 Protein; Retinoic Acid-Induced Protein 3; Phorbol Ester Induced Gene 1; Retinoic Acid Induced 3; GPCR5A; G Protein-Coupled Receptor, Class C, Group 5, Member A; Orphan G-Protein-Coupling Receptor PEIG-1; Phorbol Ester Induced Protein-1; Retinoic Acid Responsive; TPA Induced Gene 1; RAIG-1
Background

Gene Name: GPRC5A

NCBI Gene Entry: 9052

UniProt Entry: Q8NFJ5

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 40-50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2405

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human GPRC5A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000
Flow cytometry (FCM): 1:100
Immunocytochemistry (ICC): 1:100
Note This product is for research use only.
Data
  • Western blotting analysis using GPRC5A antibody (Cat#67017, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
  • Western blotting analysis using GPRC5A antibody (Cat#67017, 1:1,000). GPRC5A expression in wild-type (WT) and GPRC5A shRNA knockdown (KD) MCF7 cells of total cell lysates. GAPDH serves as a loading control.
  • Western blotting analysis using GPRC5A antibody (Cat#67017). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GPRC5A antibody (Cat#67017, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively.
  • Immunocytochemical staining of MCF7 cells using GPRC5A antibody (Cat#67017, 1:100). Top panel: wild type (WT); Bottom panel: GPRC5A shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; GPRC5A was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
  • Flow cytometric analysis of GPRC5A expression in HeLa cells using GPRC5A antibody (Cat#67017, 1:100). Red, isotype control; blue, GPRC5A.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GPRC5A
  • Catalog：
    67017
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using GPRC5A antibody (Cat#67017, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using GPRC5A antibody (Cat#67017, 1:1,000). GPRC5A expression in wild-type (WT) and GPRC5A shRNA knockdown (KD) MCF7 cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using GPRC5A antibody (Cat#67017). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GPRC5A antibody (Cat#67017, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively.
Immunocytochemical staining of MCF7 cells using GPRC5A antibody (Cat#67017, 1:100). Top panel: wild type (WT); Bottom panel: GPRC5A shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; GPRC5A was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Flow cytometric analysis of GPRC5A expression in HeLa cells using GPRC5A antibody (Cat#67017, 1:100). Red, isotype control; blue, GPRC5A.
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