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KD-Validated CD8A Rabbit pAb

#61835

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Knockdown Validated
Western blotting analysis using CD8A antibody (Cat#61835). CD8A expression in wild type (WT) and CD8A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CD8A antibody (Cat#61835, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CD8A
  • Catalog:
    61835
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated CD8A Rabbit pAb
Aliases CD8A; CD8 Subunit Alpha; T-Cell Surface Glycoprotein CD8 Alpha Chain; CD8alpha; P32; T-Lymphocyte Differentiation Antigen T8/Leu-2; CD8 Antigen, Alpha Polypeptide (P32); CD8a Molecule; CD8; Leu2 T-Lymphocyte Antigen; OKT8 T-Cell Antigen; T-Cell Antigen Leu2; T Cell Co-Receptor T8; T-Cell Antigen; CD8a Antigen; IMD116; Leu2; MAL
Background

Gene Name: CD8A

NCBI Gene Entry: 925

UniProt Entry: P01732

Application Information

Molecular Weight: Predicted, 26 kDa; observed, 45 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human CD8A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD8A antibody (Cat#61835). CD8A expression in wild type (WT) and CD8A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CD8A antibody (Cat#61835, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CD8A antibody (Cat#61835). CD8A expression in wild type (WT) and CD8A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CD8A antibody (Cat#61835, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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