KD-Validated PPWD1 Mouse mAb - Product - Hefei GenuIN Biotech Co., Ltd.

KD-Validated PPWD1 Mouse mAb

#61905

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Knockdown Validated

Western blotting analysis using PPWD1 antibody (Cat#61905). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPWD1 antibody (Cat#61905, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using PPWD1 antibody (Cat#61905). PPWD1 expression in wild-type (WT) and PPWD1 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPWD1 antibody (Cat#61905, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of PPWD1 expression in HeLa cells using PPWD1 antibody (Cat#61905, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPWD1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PPWD1
Catalog：

61905
Application：

WB, FCM
Species Reactivity：

H

Information

Data

Information

Product Name	KD-Validated PPWD1 Mouse mAb
Aliases	PPWD1; Peptidylprolyl Isomerase Domain And WD Repeat Containing 1; KIAA0073; Peptidylprolyl Isomerase Domain And WD Repeat-Containing Protein 1; Spliceosome-Associated Cyclophilin; EC 5.2.1.8; Epididymis Secretory Sperm Binding Protein
Background	Gene Name: PPWD1 NCBI Gene Entry: 23398 UniProt Entry: Q96BP3
Application Information	Molecular Weight: Predicted, 74 kDa; observed, 73 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB4130 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human PPWD1
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using PPWD1 antibody (Cat#61905). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPWD1 antibody (Cat#61905, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PPWD1 antibody (Cat#61905). PPWD1 expression in wild-type (WT) and PPWD1 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPWD1 antibody (Cat#61905, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PPWD1 expression in HeLa cells using PPWD1 antibody (Cat#61905, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPWD1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PPWD1
Catalog：

61905
Application：

WB, FCM
Species Reactivity：

H

Datasheet

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