Western blotting analysis using EYA2 antibody (Cat#61978). EYA2 expression in wild-type (WT) and EYA2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EYA2 antibody (Cat#61978, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using EYA2 antibody (Cat#61978). EYA2 expression in wild-type (WT) and EYA2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EYA2 antibody (Cat#61978, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using EYA2 antibody (Cat#61978). EYA2 expression in wild-type (WT) and EYA2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EYA2 antibody (Cat#61978, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
