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Home>Product>Primary Antibody

KD-Validated KCTD9 Recombinant Rabbit mAb

#61999

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using KCTD9 antibody (Cat#61999). KCTD9 expression in wild-type (WT) and KCTD9 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KCTD9 antibody (Cat#61999, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of KCTD9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with KCTD9 antibody (Cat#61999, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KCTD9
  • Catalog：
    61999
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated KCTD9 Recombinant Rabbit mAb
Aliases KCTD9; Potassium Channel Tetramerization Domain Containing 9; BTBD27; Potassium Channel Tetramerisation Domain Containing 9; BTB/POZ Domain-Containing Protein KCTD9; FLJ20038
Background

Gene Name: KCTD9

NCBI Gene Entry: 54793

UniProt Entry: Q7L273

Application Information

Molecular Weight: Predicted, 43 kDa; observed, 43 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7800

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human KCTD9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using KCTD9 antibody (Cat#61999). KCTD9 expression in wild-type (WT) and KCTD9 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KCTD9 antibody (Cat#61999, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of KCTD9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with KCTD9 antibody (Cat#61999, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KCTD9
  • Catalog：
    61999
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using KCTD9 antibody (Cat#61999). KCTD9 expression in wild-type (WT) and KCTD9 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KCTD9 antibody (Cat#61999, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of KCTD9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with KCTD9 antibody (Cat#61999, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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