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ALPL Mouse mAb

#6201

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Western blotting analysis using ALPL antibody (Cat#6201). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALPL antibody (Cat#6201, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ALPL expression in HAP-1 cells using ALPL antibody (Cat#6201, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ALPL.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ALPL
  • Catalog:
    6201
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name ALPL Mouse mAb
Aliases Alkaline Phosphatase, Biomineralization Associated; TNSALP; TNALP; TNAP; Alkaline Phosphatase, Tissue-Nonspecific Isozyme; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; Tissue Non-Specific Alkaline Phosphatase; Alkaline Phosphatase, Liver/Bone/Kidney; Phosphocreatine Phosphatase; Phosphoamidase; EC 3.1.3.1; AP-TNAP; TNS-ALP; HOPS; Liver/Bone/Kidney-Type Alkaline Phosphatase; Tissue-Nonspecific ALP; EC 3.9.1.1; APTNAP; HPPA; HPPC; HPPI; HPPO
Background

Gene Name: ALPL

NCBI Gene Entry: 249

UniProt Entry: P05186

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 75 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB7580

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human ALPL
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using ALPL antibody (Cat#6201). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALPL antibody (Cat#6201, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of ALPL expression in HAP-1 cells using ALPL antibody (Cat#6201, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ALPL.
Western blotting analysis using ALPL antibody (Cat#6201). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALPL antibody (Cat#6201, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ALPL expression in HAP-1 cells using ALPL antibody (Cat#6201, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ALPL.
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