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KD-Validated PSME1 Mouse mAb

#62127

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shRNA Knockdown Validated
Western blotting analysis using PSME1 antibody (Cat#62127). PSME1 expression in wild-type (WT) and PSME1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PSME1 antibody (Cat#62127, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PSME1
  • Catalog:
    62127
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated PSME1 Mouse mAb
Aliases PSME1; Proteasome Activator Subunit 1; PA28alpha; IFI5111; Proteasome (Prosome, Macropain) Activator Subunit 1 (PA28 Alpha); Activator Of Multicatalytic Protease Subunit 1; Interferon Gamma Up-Regulated I-5111 Protein; Proteasome Activator Complex Subunit 1; 11S Regulator Complex Subunit Alpha; IGUP I-5111; Epididymis Secretory Sperm Binding Protein Li 129m; Interferon-Gamma-Inducible Protein 5111; Proteasome Activator 28 Subunit Alpha; Interferon-Gamma IEF SSP 5111; 29-KD MCP Activator Subunit; HEL-S-129m; REG-Alpha; REGalpha; PA28A; PA28a
Background

Gene Name: PSME1

NCBI Gene Entry: 5720

UniProt Entry: Q06323

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 29 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1495

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human PSME1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PSME1 antibody (Cat#62127). PSME1 expression in wild-type (WT) and PSME1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PSME1 antibody (Cat#62127, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PSME1 antibody (Cat#62127). PSME1 expression in wild-type (WT) and PSME1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PSME1 antibody (Cat#62127, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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