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Home>Product>Primary Antibody

KD-Validated Adenylate Kinase 1 Mouse mAb

#62872

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shRNA Knockdown Validated
Western blotting analysis using adenylate kinase 1 antibody (Cat#62872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using adenylate kinase 1 antibody (Cat#62872). Adenylate kinase 1 expression in wild-type (WT) and adenylate kinase 1 (AK1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AK1
  • Catalog：
    62872
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Adenylate Kinase 1 Mouse mAb
Aliases AK1; Adenylate Kinase 1; Adenylate Monophosphate Kinase; Adenylate Kinase Isoenzyme 1; ATP-AMP Transphosphorylase 1; ATP:AMP Phosphotransferase; EC 2.7.4.3; Myokinase; Testis Secretory Sperm Binding Protein Li 58j; Epididymis Secretory Sperm Binding Protein; EC 2.7.4.10; EC 2.7.4.6; HTL-S-58j; EC 2.7.4; AK 1
Background

Gene Name: AK1

NCBI Gene Entry: 203

UniProt Entry: P00568

Application Information

Molecular Weight: Predicted, 22 kDa; observed, 22 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2940

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human Adenylate Kinase 1
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using adenylate kinase 1 antibody (Cat#62872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using adenylate kinase 1 antibody (Cat#62872). Adenylate kinase 1 expression in wild-type (WT) and adenylate kinase 1 (AK1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AK1
  • Catalog：
    62872
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using adenylate kinase 1 antibody (Cat#62872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using adenylate kinase 1 antibody (Cat#62872). Adenylate kinase 1 expression in wild-type (WT) and adenylate kinase 1 (AK1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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