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Home>Product>Primary Antibody

KD-Validated ATP6V1A Recombinant Rabbit mAb

#63693

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using ATP6V1A antibody (Cat#63693). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP6V1A antibody (Cat#63693, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ATP6V1A antibody (Cat#63693). ATP6V1A expression in wild-type (WT) and ATP6V1A shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP6V1A antibody (Cat#63693, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using ATP6V1A antibody (Cat#63693, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with ATP6V1A antibody (Cat#63693, 1:1,000). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HT-1080 cells using ATP6V1A antibody (Cat#63693, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP6V1A shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of ATP6V1A expression in HepG2 cells using ATP6V1A antibody (Cat#63693, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ATP6V1A.
Validation of ATP6V1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with ATP6V1A antibody (Cat#63693, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP6V1A
  • Catalog：
    63693
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ATP6V1A Recombinant Rabbit mAb
Aliases ATP6V1A; ATPase H+ Transporting V1 Subunit A; V-ATPase Subunit A; ATP6V1A1; ATP6A1; Vma1; VA68; VPP2; ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A; V-Type Proton ATPase (V-ATPase) Catalytic Subunit A; V-Type Proton ATPase Catalytic Subunit A; Vacuolar Proton Pump Subunit Alpha; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump), Alpha Polypeptide, 70kD, Isoform 1; H+-Transporting ATPase Chain A, Vacuolar (VA68 Type); ATPase, H+ Transporting, Lysosomal, Subunit A1; H(+)-Transporting Two-Sector ATPase, Subunit A; Vacuolar Proton Pump Alpha Subunit 1; Vacuolar ATPase Isoform VA68; V-ATPase 69 KDa Subunit 1; Vacuolar-Type H(+)-ATPase; V-ATPase 69 KDa Subunit; V-ATPase A Subunit 1; EC 3.6.3.14; EC 7.1.2.2; EC 3.6.3; ARCL2D; IECEE3; DEE93; HO68
Background

Gene Name: ATP6V1A

NCBI Gene Entry: 523

UniProt Entry: P38606

Application Information

Molecular Weight: Predicted, 68 kDa; observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6940

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human ATP6V1A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using ATP6V1A antibody (Cat#63693). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP6V1A antibody (Cat#63693, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ATP6V1A antibody (Cat#63693). ATP6V1A expression in wild-type (WT) and ATP6V1A shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP6V1A antibody (Cat#63693, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using ATP6V1A antibody (Cat#63693, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with ATP6V1A antibody (Cat#63693, 1:1,000). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HT-1080 cells using ATP6V1A antibody (Cat#63693, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP6V1A shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of ATP6V1A expression in HepG2 cells using ATP6V1A antibody (Cat#63693, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ATP6V1A.
  • Validation of ATP6V1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with ATP6V1A antibody (Cat#63693, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP6V1A
  • Catalog：
    63693
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATP6V1A antibody (Cat#63693). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP6V1A antibody (Cat#63693, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ATP6V1A antibody (Cat#63693). ATP6V1A expression in wild-type (WT) and ATP6V1A shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP6V1A antibody (Cat#63693, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using ATP6V1A antibody (Cat#63693, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with ATP6V1A antibody (Cat#63693, 1:1,000). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HT-1080 cells using ATP6V1A antibody (Cat#63693, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP6V1A shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of ATP6V1A expression in HepG2 cells using ATP6V1A antibody (Cat#63693, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ATP6V1A.
Validation of ATP6V1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with ATP6V1A antibody (Cat#63693, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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