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Home>Product>Primary Antibody

KD-Validated Sorting Nexin 9 Mouse mAb

#64838

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shRNA Knockdown Validated
Western blotting analysis using SNX9 antibody (Cat#64838). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SNX9 antibody (Cat#64838, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SNX9 antibody (Cat#64838). SNX9 expression in wild-type (WT) and SNX9 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SNX9 antibody (Cat#64838, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with SNX9 antibody (Cat#64838, 1:1,000). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using SNX9 antibody (Cat#64838, 1:1,000), Top panel: wild-type (WT); Bottom panal: SNX9 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of SNX9 expression in HeLa cells using SNX9 antibody (Cat#64838, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SNX9.
Validation of SNX9 knockdown using flow cytometry. Wild-type(WT, Blue) and SNX9 knockdown(KD, Green) HeLa cells were stained with SNX9 antibody(Cat#64838, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SNX9
  • Catalog：
    64838
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Sorting Nexin 9 Mouse mAb
Aliases SNX9; Sorting Nexin 9; SH3PXD3A; SH3PX1; SDP1; SH3 And PX Domain-Containing Protein 3A; SH3 And PX Domain-Containing Protein 1; Sorting Nexin-9; Wiskott-Aldrich Syndrome Protein (WASP) Interactor Protein; Protein SDP1; WISP
Background

Gene Name: SNX9

NCBI Gene Entry: 51429

UniProt Entry: Q9Y5X1

Application Information

Molecular Weight: Predicted, 67 kDa; observed, 73 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13195

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human SNX9
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SNX9 antibody (Cat#64838). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SNX9 antibody (Cat#64838, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using SNX9 antibody (Cat#64838). SNX9 expression in wild-type (WT) and SNX9 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SNX9 antibody (Cat#64838, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with SNX9 antibody (Cat#64838, 1:1,000). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HeLa cells using SNX9 antibody (Cat#64838, 1:1,000), Top panel: wild-type (WT); Bottom panal: SNX9 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of SNX9 expression in HeLa cells using SNX9 antibody (Cat#64838, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SNX9.
  • Validation of SNX9 knockdown using flow cytometry. Wild-type(WT, Blue) and SNX9 knockdown(KD, Green) HeLa cells were stained with SNX9 antibody(Cat#64838, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SNX9
  • Catalog：
    64838
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using SNX9 antibody (Cat#64838). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SNX9 antibody (Cat#64838, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SNX9 antibody (Cat#64838). SNX9 expression in wild-type (WT) and SNX9 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SNX9 antibody (Cat#64838, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with SNX9 antibody (Cat#64838, 1:1,000). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using SNX9 antibody (Cat#64838, 1:1,000), Top panel: wild-type (WT); Bottom panal: SNX9 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of SNX9 expression in HeLa cells using SNX9 antibody (Cat#64838, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SNX9.
Validation of SNX9 knockdown using flow cytometry. Wild-type(WT, Blue) and SNX9 knockdown(KD, Green) HeLa cells were stained with SNX9 antibody(Cat#64838, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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