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Home>Product>Primary Antibody

KD-Validated PAICS Mouse mAb

#64841

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shRNA Knockdown Validated
Western blotting analysis using PAICS antibody (Cat#64841). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PAICS antibody (Cat#64841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PAICS antibody (Cat#64841). PAICS expression in wild-type (WT) and PAICS shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PAICS antibody (Cat#64841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PAICS expression in HAP-1 cells using PAICS antibody (Cat#64841, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PAICS.
Validation of PAICS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PAICS antibody (Cat#64841, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PAICS
  • Catalog：
    64841
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated PAICS Mouse mAb
Aliases PAICS; Phosphoribosylaminoimidazole Carboxylase And Phosphoribosylaminoimidazolesuccinocarboxamide Synthase; ADE2H1; PAIS; Bifunctional Phosphoribosylaminoimidazole Carboxylase/Phosphoribosylaminoimidazole Succinocarboxamide Synthetase; Phosphoribosylaminoimidazole Carboxylase, Phosphoribosylaminoimidazole Succinocarboxamide Synthetase; ADE2; Phosphoribosylaminoimidazole Carboxylase; Phosphoribosylaminoimidazolesuccinocarboxamide Synthase; Multifunctional Protein ADE2H1; Multifunctional Protein ADE2; SAICAR Synthetase; AIR Carboxylase; PAICSD
Background

Gene Name: PAICS

NCBI Gene Entry: 10606

UniProt Entry: P22234

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 43 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13210

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human PAICS
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PAICS antibody (Cat#64841). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PAICS antibody (Cat#64841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using PAICS antibody (Cat#64841). PAICS expression in wild-type (WT) and PAICS shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PAICS antibody (Cat#64841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PAICS expression in HAP-1 cells using PAICS antibody (Cat#64841, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PAICS.
  • Validation of PAICS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PAICS antibody (Cat#64841, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PAICS
  • Catalog：
    64841
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using PAICS antibody (Cat#64841). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PAICS antibody (Cat#64841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PAICS antibody (Cat#64841). PAICS expression in wild-type (WT) and PAICS shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PAICS antibody (Cat#64841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PAICS expression in HAP-1 cells using PAICS antibody (Cat#64841, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PAICS.
Validation of PAICS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PAICS antibody (Cat#64841, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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