Western blotting analysis using diacylglycerol kinase alpha antibody (Cat#64923). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with diacylglycerol kinase alpha antibody (Cat#64923, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using diacylglycerol kinase alpha antibody (Cat#64923). Diacylglycerol kinase alpha expression in wild-type (WT) and diacylglycerol kinase alpha (DGKA) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with diacylglycerol kinase alpha antibody (Cat#64923, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Diacylglycerol kinase alpha expression in HepG2 cells using Diacylglycerol kinase alpha antibody (Cat#64923, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Diacylglycerol kinase alpha.
