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Home>Product>Primary Antibody

KD-Validated HNRNPH1 Mouse mAb

#65063

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shRNA Knockdown Validated
Western blotting analysis using HNRNPH1 antibody (Cat#65063). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HNRNPH1 antibody (Cat#65063, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HNRNPH1 antibody (Cat#65063). HNRNPH1 expression in wild-type (WT) and HNRNPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HNRNPH1 antibody (Cat#65063, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HNRNPH1 expression in HeLa cells using HNRNPH1 antibody (Cat#65063, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS. Green, isotype control; red, HNRNPH1.
Validation of HNRNPH1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HNRNPH1 antibody (Cat#65063, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    HNRNPH1
  • Catalog：
    65063
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated HNRNPH1 Mouse mAb
Aliases HNRNPH1; Heterogeneous Nuclear Ribonucleoprotein H1; Heterogeneous Nuclear Ribonucleoprotein H; HnRNPH; HNRPH1; Heterogeneous Nuclear Ribonucleoprotein H1 (H); HNRPH; Epididymis Secretory Sperm Binding Protein; HnRNP H; NEDCDS
Background

Gene Name: HNRNPH1

NCBI Gene Entry: 3187

UniProt Entry: P31943

Application Information

Molecular Weight: Predicted, 49 kDa; observed, 49 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB14105

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human HNRNPH1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using HNRNPH1 antibody (Cat#65063). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HNRNPH1 antibody (Cat#65063, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using HNRNPH1 antibody (Cat#65063). HNRNPH1 expression in wild-type (WT) and HNRNPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HNRNPH1 antibody (Cat#65063, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of HNRNPH1 expression in HeLa cells using HNRNPH1 antibody (Cat#65063, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS. Green, isotype control; red, HNRNPH1.
  • Validation of HNRNPH1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HNRNPH1 antibody (Cat#65063, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HNRNPH1
  • Catalog：
    65063
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using HNRNPH1 antibody (Cat#65063). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HNRNPH1 antibody (Cat#65063, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HNRNPH1 antibody (Cat#65063). HNRNPH1 expression in wild-type (WT) and HNRNPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HNRNPH1 antibody (Cat#65063, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HNRNPH1 expression in HeLa cells using HNRNPH1 antibody (Cat#65063, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS. Green, isotype control; red, HNRNPH1.
Validation of HNRNPH1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HNRNPH1 antibody (Cat#65063, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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