Western blotting analysis using argininosuccinate synthase 1 antibody (Cat#65873). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with argininosuccinate synthase 1 antibody (Cat#65873, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using argininosuccinate synthase 1 antibody (Cat#65873). Argininosuccinate synthase 1 expression in wild-type (WT) and argininosuccinate synthase 1 (ASS1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with argininosuccinate synthase 1 antibody (Cat#65873, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Argininosuccinate synthase 1 expression in HepG2 cells using Argininosuccinate synthase 1 antibody (Cat#65873, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Argininosuccinate synthase 1.
