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Home>Product>Primary Antibody

Enolase 1 Mouse mAb

#6596

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Western blotting analysis using enolase 1 antibody (Cat#6596). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with enolase 1 antibody (Cat#6596, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Enolase 1 antibody (Cat#6596, 1:1,000). Nuclei were stained blue with DAPI; Enolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Enolase 1 expression in HepG2 cells using Enolase 1 antibody (Cat#6596, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Enolase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ENO1
  • Catalog：
    6596
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Enolase 1 Mouse mAb
Aliases ENO1; Enolase 1; Alpha-Enolase; ENO1L1; MBP-1; MPB1; PPH; ENO1 Intronic Transcript 1 (Non-Protein Coding); 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; ENO1 Intronic Transcript 1; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; EC 4.2.1.11; ENO1-IT1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1; MYC Promoter-Binding Protein 1; C-Myc Promoter-Binding Protein; Alpha Enolase Like 1; Tau-Crystallin; Enolase-Alpha; HEL-S-17; EC 4.2.1; MBPB1; MPB-1
Background

Gene Name: ENO1

NCBI Gene Entry: 2023

UniProt Entry: P06733

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 47 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB55

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human ENO1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using enolase 1 antibody (Cat#6596). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with enolase 1 antibody (Cat#6596, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Enolase 1 antibody (Cat#6596, 1:1,000). Nuclei were stained blue with DAPI; Enolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Enolase 1 expression in HepG2 cells using Enolase 1 antibody (Cat#6596, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Enolase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ENO1
  • Catalog：
    6596
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using enolase 1 antibody (Cat#6596). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with enolase 1 antibody (Cat#6596, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Enolase 1 antibody (Cat#6596, 1:1,000). Nuclei were stained blue with DAPI; Enolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Enolase 1 expression in HepG2 cells using Enolase 1 antibody (Cat#6596, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Enolase 1.
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