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Home>Product>Primary Antibody

KD-Validated PCCA Mouse mAb

#66110

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shRNA Knockdown Validated
Western blotting analysis using PCCA antibody (Cat#66110). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PCCA antibody (Cat#66110, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PCCA antibody (Cat#66110). PCCA expression in wild-type (WT) and PCCA shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PCCA antibody (Cat#66110, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with PCCA antibody (Cat#66110, 1:1,000). Nuclei were stained blue with DAPI; PCCA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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  • Gene Symbol：
    PCCA
  • Catalog：
    66110
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated PCCA Mouse mAb
Aliases PCCA; Propionyl-CoA Carboxylase Subunit Alpha; Propionyl-CoA Carboxylase Alpha Chain, Mitochondrial; Propionyl Coenzyme A Carboxylase, Alpha Polypeptide; EC 6.4.1.3; Propanoyl-CoA:Carbon Dioxide Ligase Alpha Subunit; Propanoyl-CoA:Carbon Dioxide Ligase Subunit Alpha; Propionyl CoA Carboxylase, Alpha Polypeptide; Propionyl-CoA Carboxylase Alpha Subunit; PccA Complementation Group; PCCase Alpha Subunit; PCCase Subunit Alpha; EC 6.4.1
Background

Gene Name: PCCA

NCBI Gene Entry: 5095

UniProt Entry: P05165

Application Information

Molecular Weight: Predicted, 80 kDa; observed, 70 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB6905

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PCCA
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PCCA antibody (Cat#66110). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PCCA antibody (Cat#66110, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using PCCA antibody (Cat#66110). PCCA expression in wild-type (WT) and PCCA shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PCCA antibody (Cat#66110, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with PCCA antibody (Cat#66110, 1:1,000). Nuclei were stained blue with DAPI; PCCA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PCCA
  • Catalog：
    66110
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PCCA antibody (Cat#66110). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PCCA antibody (Cat#66110, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PCCA antibody (Cat#66110). PCCA expression in wild-type (WT) and PCCA shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PCCA antibody (Cat#66110, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with PCCA antibody (Cat#66110, 1:1,000). Nuclei were stained blue with DAPI; PCCA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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