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Home>Product>Primary Antibody

KD-Validated Cyclin D1 Recombinant Rabbit mAb

#69541

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Cyclin D1 antibody (Cat#69541). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Cyclin D1 antibody (Cat#69541). Cyclin D1 expression in wild type (WT) and Cyclin D1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Cyclin D1 antibody (Cat#69541, 1:1,000). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using Cyclin D1 antibody (Cat#69541, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cyclin D1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Cyclin D1 expression in C2C12 cells using Cyclin D1 antibody (Cat#69541, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cyclin D1.
Validation of Cyclin D1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Cyclin D1 antibody (Cat#69541, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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  • Gene Symbol：
    CCND1
  • Catalog：
    69541
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Cyclin D1 Recombinant Rabbit mAb
Aliases CCND1; Cyclin D1; U21B31; PRAD1; BCL1; B-Cell Lymphoma 1 Protein; G1/S-Specific Cyclin-D1; B-Cell CLL/Lymphoma 1; BCL-1 Oncogene; PRAD1 Oncogene; D11S287E; Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1); Parathyroid Adenomatosis 1; G1/S-Specific Cyclin D1; BCL-1
Background

Gene Name: CCND1

NCBI Gene Entry: 595

UniProt Entry: P24385

Application Information

Molecular Weight: Predicted, 34 kDa; observed, 34 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB735

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Cyclin D1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Cyclin D1 antibody (Cat#69541). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Cyclin D1 antibody (Cat#69541). Cyclin D1 expression in wild type (WT) and Cyclin D1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Cyclin D1 antibody (Cat#69541, 1:1,000). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using Cyclin D1 antibody (Cat#69541, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cyclin D1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of Cyclin D1 expression in C2C12 cells using Cyclin D1 antibody (Cat#69541, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cyclin D1.
  • Validation of Cyclin D1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Cyclin D1 antibody (Cat#69541, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CCND1
  • Catalog：
    69541
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Cyclin D1 antibody (Cat#69541). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Cyclin D1 antibody (Cat#69541). Cyclin D1 expression in wild type (WT) and Cyclin D1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Cyclin D1 antibody (Cat#69541, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Cyclin D1 antibody (Cat#69541, 1:1,000). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using Cyclin D1 antibody (Cat#69541, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cyclin D1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Cyclin D1 expression in C2C12 cells using Cyclin D1 antibody (Cat#69541, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cyclin D1.
Validation of Cyclin D1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Cyclin D1 antibody (Cat#69541, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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