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Home>Product>Primary Antibody

KO-Validated Phospho-IGF1 Receptor (Y1166) Recombinant Rabbit mAb

#71265

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265). phospho-IGF1 Receptor (Tyr1166) expression in wild-type (WT) and IGF1R knockout (KO) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-IGF1 Receptor (Tyr1166) expression in HAP-1 cells using Phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-IGF1 Receptor (Tyr1166).
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  • Gene Symbol：
    IGF1R
  • Catalog：
    71265
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KO-Validated Phospho-IGF1 Receptor (Y1166) Recombinant Rabbit mAb
Aliases IGF1R; Insulin Like Growth Factor 1 Receptor; Insulin-Like Growth Factor 1 Receptor; JTK13; CD221; IGFIR; IGFR; IGF-I Receptor; EC 2.7.10.1; MGC18216; Insulin-Like Growth Factor I Receptor; CD221 Antigen; EC 2.7.10
Background

Gene Name: IGF1R

NCBI Gene Entry: 3480

UniProt Entry: P08069

Application Information

Molecular Weight: Predicted, 155 kDa; observed, 95 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4080

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human Phospho-IGF1 Receptor (Tyr1166)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265). phospho-IGF1 Receptor (Tyr1166) expression in wild-type (WT) and IGF1R knockout (KO) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Phospho-IGF1 Receptor (Tyr1166) expression in HAP-1 cells using Phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-IGF1 Receptor (Tyr1166).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IGF1R
  • Catalog：
    71265
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265). phospho-IGF1 Receptor (Tyr1166) expression in wild-type (WT) and IGF1R knockout (KO) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-IGF1 Receptor (Tyr1166) expression in HAP-1 cells using Phospho-IGF1 Receptor (Tyr1166) antibody (Cat#71265, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-IGF1 Receptor (Tyr1166).
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