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Home>Product>Primary Antibody

AGTR1 Recombinant Rabbit mAb

#9371

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Recombinant Antibody
Western blotting analysis using AGTR1 antibody (Cat#9371). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AGTR1 antibody (Cat#9371, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with AGTR1 antibody (Cat#9371, 1:1,000). Nuclei were stained blue with DAPI; AGTR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of AGTR1 expression in HAP-1 cells using AGTR1 antibody (Cat#9371, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AGTR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AGTR1
  • Catalog：
    9371
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name AGTR1 Recombinant Rabbit mAb
Aliases AGTR1; Angiotensin II Receptor Type 1; AT2R1; AGTR1A; AT2R1B; AGTR1B; HAT1R; AG2S; AT1B; AT1; Type-1 Angiotensin II Receptor; AT1 Receptor; AT2R1A; AT1AR; AT1BR; ATR1; Type-1B Angiotensin II Receptor; Angiotensin II Type 1 Receptor; Angiotensin II Type-1 Receptor; Angiotensin Receptor 1B; AT1R
Background

Gene Name: AGTR1

NCBI Gene Entry: 185

UniProt Entry: P30556

Application Information

Molecular Weight: Predicted, 41 kDa; observed, 41 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB635

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human AGTR1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using AGTR1 antibody (Cat#9371). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AGTR1 antibody (Cat#9371, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with AGTR1 antibody (Cat#9371, 1:1,000). Nuclei were stained blue with DAPI; AGTR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of AGTR1 expression in HAP-1 cells using AGTR1 antibody (Cat#9371, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AGTR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AGTR1
  • Catalog：
    9371
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using AGTR1 antibody (Cat#9371). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AGTR1 antibody (Cat#9371, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with AGTR1 antibody (Cat#9371, 1:1,000). Nuclei were stained blue with DAPI; AGTR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of AGTR1 expression in HAP-1 cells using AGTR1 antibody (Cat#9371, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AGTR1.
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