Western blotting analysis using Phospho-ERK1 (T202) + ERK2 (T185) antibody (Cat#1798). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-ERK1 (T202) + ERK2 (T185) antibody (Cat#1798, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-ERK1 (T202) + ERK2 (T185) antibody (Cat#1798, 1:1,000). Nuclei were stained blue with DAPI; Phospho-ERK1 (T202) + ERK2 (T185) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using Phospho-ERK1 (T202) + ERK2 (T185) antibody (Cat#1798). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-ERK1 (T202) + ERK2 (T185) antibody (Cat#1798, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-ERK1 (T202) + ERK2 (T185) antibody (Cat#1798, 1:1,000). Nuclei were stained blue with DAPI; Phospho-ERK1 (T202) + ERK2 (T185) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using Phospho-ERK1 (T202) + ERK2 (T185) antibody (Cat#1798). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-ERK1 (T202) + ERK2 (T185) antibody (Cat#1798, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-ERK1 (T202) + ERK2 (T185) antibody (Cat#1798, 1:1,000). Nuclei were stained blue with DAPI; Phospho-ERK1 (T202) + ERK2 (T185) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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