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S-Phase Kinase Associated Protein 1 Recombinant Rabbit mAb

#1701

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Recombinant Antibody
Western blotting analysis using S-phase kinase associated protein 1 antibody (Cat#1701). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with S-phase kinase associated protein 1 antibody (Cat#1701, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with S-phase kinase associated protein 1 antibody (Cat#1701, 1:1,000). Nuclei were stained blue with DAPI; S-phase kinase associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of S-phase kinase associated protein 1 expression in HeLa cells using S-phase kinase associated protein 1 antibody (Cat#1701, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, S-phase kinase associated protein 1.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    SKP1
  • Catalog:
    1701
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name S-Phase Kinase Associated Protein 1 Recombinant Rabbit mAb
Aliases SKP1; S-Phase Kinase Associated Protein 1; TCEB1L; OCP-II; EMC19; OCP2; P19A; SKP1A; Transcription Elongation Factor B Polypeptide 1-Like; Cyclin-A/CDK2-Associated Protein P19; S-Phase Kinase-Associated Protein 1; Organ Of Corti Protein II; Organ Of Corti Protein; MGC34403; P19skp1; OCP-2; SIII; RNA Polymerase II Elongation Factor-Like Protein OCP2; RNA Polymerase II Elongation Factor-Like Protein; S-Phase Kinase-Associated Protein 1A (P19A); Cyclin A/CDK2-Associated P19
Background

Gene Name: SKP1

NCBI Gene Entry: 6500

UniProt Entry: P63208

Application Information

Molecular Weight: Predicted, 19 kDa; observed, 19 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4140

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human Skp1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using S-phase kinase associated protein 1 antibody (Cat#1701). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with S-phase kinase associated protein 1 antibody (Cat#1701, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells with S-phase kinase associated protein 1 antibody (Cat#1701, 1:1,000). Nuclei were stained blue with DAPI; S-phase kinase associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of S-phase kinase associated protein 1 expression in HeLa cells using S-phase kinase associated protein 1 antibody (Cat#1701, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, S-phase kinase associated protein 1.
Western blotting analysis using S-phase kinase associated protein 1 antibody (Cat#1701). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with S-phase kinase associated protein 1 antibody (Cat#1701, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with S-phase kinase associated protein 1 antibody (Cat#1701, 1:1,000). Nuclei were stained blue with DAPI; S-phase kinase associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of S-phase kinase associated protein 1 expression in HeLa cells using S-phase kinase associated protein 1 antibody (Cat#1701, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, S-phase kinase associated protein 1.
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