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Home>Product>Primary Antibody

Lamin B Receptor Recombinant Rabbit mAb

#2121

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Recombinant Antibody
Western blotting analysis using Lamin B receptor antibody (Cat#2121). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Lamin B receptor antibody (Cat#2121, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Lamin B receptor antibody (Cat#2121, 1:1,000). Nuclei were stained blue with DAPI;Lamin B receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Lamin B receptor expression in HAP-1 cells using Lamin B receptor antibody (Cat#2121, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Lamin B receptor.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LBR
  • Catalog：
    2121
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name Lamin B Receptor Recombinant Rabbit mAb
Aliases LBR; Lamin B Receptor; DHCR14B; TDRD18; Integral Nuclear Envelope Inner Membrane Protein; 3-Beta-Hydroxysterol Delta (14)-Reductase; Delta(14)-Sterol Reductase LBR; Tudor Domain Containing 18; C-14 Sterol Reductase; Sterol C14-Reductase; Delta-14-SR; C14SR; LMN2R; Lamin-B Receptor; EC 1.3.1.70; PHASK; PHA
Background

Gene Name: LBR

NCBI Gene Entry: 3930

UniProt Entry: Q14739

Application Information

Molecular Weight: Predicted, 71 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6100

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Lamin B Receptor
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Lamin B receptor antibody (Cat#2121). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Lamin B receptor antibody (Cat#2121, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with Lamin B receptor antibody (Cat#2121, 1:1,000). Nuclei were stained blue with DAPI;Lamin B receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Lamin B receptor expression in HAP-1 cells using Lamin B receptor antibody (Cat#2121, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Lamin B receptor.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LBR
  • Catalog：
    2121
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using Lamin B receptor antibody (Cat#2121). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Lamin B receptor antibody (Cat#2121, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Lamin B receptor antibody (Cat#2121, 1:1,000). Nuclei were stained blue with DAPI;Lamin B receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Lamin B receptor expression in HAP-1 cells using Lamin B receptor antibody (Cat#2121, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Lamin B receptor.
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