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Home>Product>Primary Antibody

MAP2K2 Recombinant Rabbit mAb

#2951

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Recombinant Antibody
Western blotting analysis using MAP2K2 antibody (Cat#2951). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP2K2 antibody (Cat#2951, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MAP2K2 antibody (Cat#2951, 1:1,000). Nuclei were stained blue with DAPI; MAP2K2 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MAP2K2 expression in HepG2 cells using MAP2K2 antibody (Cat#2951, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAP2K2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAP2K2
  • Catalog：
    2951
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name MAP2K2 Recombinant Rabbit mAb
Aliases MAP2K2; Mitogen-Activated Protein Kinase Kinase 2; MEK2; MAP Kinase Kinase 2; PRKMK2; MKK2; Dual Specificity Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; Mitogen-Activated Protein Kinase Kinase 2, P45; MAPKK 2; MAPKK2; MEK 2; CFC4
Background

Gene Name: MAP2K2

NCBI Gene Entry: 5605

UniProt Entry: P36507

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 44 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3320

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human MEK2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MAP2K2 antibody (Cat#2951). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP2K2 antibody (Cat#2951, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with MAP2K2 antibody (Cat#2951, 1:1,000). Nuclei were stained blue with DAPI; MAP2K2 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of MAP2K2 expression in HepG2 cells using MAP2K2 antibody (Cat#2951, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAP2K2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAP2K2
  • Catalog：
    2951
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using MAP2K2 antibody (Cat#2951). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP2K2 antibody (Cat#2951, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MAP2K2 antibody (Cat#2951, 1:1,000). Nuclei were stained blue with DAPI; MAP2K2 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MAP2K2 expression in HepG2 cells using MAP2K2 antibody (Cat#2951, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAP2K2.
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