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Home>Product>Primary Antibody

PFKFB3 Recombinant Rabbit mAb

#4399

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Recombinant Antibody
Western blotting analysis using PFKFB3 antibody (Cat#4399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PFKFB3 antibody (Cat#4399, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with PFKFB3 antibody (Cat#4399, 1:1,000). Nuclei were stained blue with DAPI; PFKFB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PFKFB3 expression in HT-1080 cells using PFKFB3 antibody (Cat#4399, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PFKFB3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PFKFB3
  • Catalog：
    4399
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name PFKFB3 Recombinant Rabbit mAb
Aliases PFKFB3; 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3; 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase Brain/Placenta-Type Isozyme 3; Renal Carcinoma Antigen NY-REN-56; 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3; IPFK-2; Inducible 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase; Fructose-6-Phosphate,2-Kinase/Fructose-2, 6-Bisphosphatase; 6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase; IPFK2; PFK2
Background

Gene Name: PFKFB3

NCBI Gene Entry: 5209

UniProt Entry: Q16875

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11025

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human PFKFB3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PFKFB3 antibody (Cat#4399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PFKFB3 antibody (Cat#4399, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with PFKFB3 antibody (Cat#4399, 1:1,000). Nuclei were stained blue with DAPI; PFKFB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PFKFB3 expression in HT-1080 cells using PFKFB3 antibody (Cat#4399, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PFKFB3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PFKFB3
  • Catalog：
    4399
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using PFKFB3 antibody (Cat#4399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PFKFB3 antibody (Cat#4399, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with PFKFB3 antibody (Cat#4399, 1:1,000). Nuclei were stained blue with DAPI; PFKFB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PFKFB3 expression in HT-1080 cells using PFKFB3 antibody (Cat#4399, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PFKFB3.
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