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BDH2 Mouse mAb

#5394

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Western blotting analysis using 3-hydroxybutyrate dehydrogenase 2 antibody (Cat#5394). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with 3-hydroxybutyrate dehydrogenase 2 antibody (Cat#5394, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of 3-hydroxybutyrate dehydrogenase 2 expression in HepG2 cells using 3-hydroxybutyrate dehydrogenase 2 antibody (Cat#5394, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, 3-hydroxybutyrate dehydrogenase 2.
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Unit price:
$69.00
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  • Gene Symbol:
    BDH2
  • Catalog:
    5394
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name BDH2 Mouse mAb
Aliases BDH2; 3-Hydroxybutyrate Dehydrogenase 2; SDR15C1; PRO20933; UCPA-OR; UNQ6308; DHRS6; Short Chain Dehydrogenase/Reductase Family 15C Member 1; Dehydrogenase/Reductase SDR Family Member 6; 3-Hydroxybutyrate Dehydrogenase, Type 2; (R)-Beta-Hydroxybutyrate Dehydrogenase; 4-Oxo-L-Proline Reductase; Oxidoreductase UCPA; FLJ13261; Short Chain Dehydrogenase/Reductase Family 15C, Member 1; Dehydrogenase/Reductase (SDR Family) Member 6; 3-Hydroxybutyrate Dehydrogenase Type 2; R-Beta-Hydroxybutyrate Dehydrogenase; EC 1.1.1.104; EC 1.1.1.30; EC 1.1.1.-; EFA6R
Background

Gene Name: BDH2

NCBI Gene Entry: 56898

UniProt Entry: Q9BUT1

Application Information

Molecular Weight: Predicted, 27 kDa; observed, 27 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1300

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human BDH2
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using 3-hydroxybutyrate dehydrogenase 2 antibody (Cat#5394). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with 3-hydroxybutyrate dehydrogenase 2 antibody (Cat#5394, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of 3-hydroxybutyrate dehydrogenase 2 expression in HepG2 cells using 3-hydroxybutyrate dehydrogenase 2 antibody (Cat#5394, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, 3-hydroxybutyrate dehydrogenase 2.
Western blotting analysis using 3-hydroxybutyrate dehydrogenase 2 antibody (Cat#5394). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with 3-hydroxybutyrate dehydrogenase 2 antibody (Cat#5394, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of 3-hydroxybutyrate dehydrogenase 2 expression in HepG2 cells using 3-hydroxybutyrate dehydrogenase 2 antibody (Cat#5394, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, 3-hydroxybutyrate dehydrogenase 2.
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