KD-Validated PRKAG1 Recombinant Rabbit mAb - Product

KD-Validated PRKAG1 Recombinant Rabbit mAb

#61582

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Recombinant Antibody
Knockdown Validated

Western blotting analysis using AMPK gamma 1 antibody (Cat#61582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AMPK gamma 1 antibody (Cat#61582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using AMPK gamma 1 antibody (Cat#61582). AMPK gamma 1 expression in wild type (WT) and PRKAG1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AMPK gamma 1 antibody (Cat#61582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using AMPK gamma 1 antibody (Cat#61582). AMPK gamma 1 expression in wild-type (WT) and PRKAG1 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AMPK gamma 1 antibody (Cat#61582, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PRKAG1
Catalog：

61582
Application：

WB
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated PRKAG1 Recombinant Rabbit mAb
Aliases	PRKAG1; Protein Kinase AMP-Activated Non-Catalytic Subunit Gamma 1; Protein Kinase, AMP-Activated, Gamma 1 Non-Catalytic Subunit; 5'-AMP-Activated Protein Kinase Subunit Gamma-1; AMPK Gamma1; 5'-AMP-Activated Protein Kinase, Gamma-1 Subunit; AMPK Subunit Gamma-1; AMPK Gamma-1 Chain; AMPKG; AMPKg
Background	Gene Name: PRKAG1 NCBI Gene Entry: 5571 UniProt Entry: P54619
Application Information	Molecular Weight: Predicted, 38 kDa; observed, 36 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB2915 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB)
Immunogen	A synthesized peptide derived from human AMPK gamma 1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western blotting (WB): 1:1,000-1:5,000
Note	This product is for research use only.

Data

Western blotting analysis using AMPK gamma 1 antibody (Cat#61582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AMPK gamma 1 antibody (Cat#61582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AMPK gamma 1 antibody (Cat#61582). AMPK gamma 1 expression in wild type (WT) and PRKAG1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AMPK gamma 1 antibody (Cat#61582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AMPK gamma 1 antibody (Cat#61582). AMPK gamma 1 expression in wild-type (WT) and PRKAG1 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AMPK gamma 1 antibody (Cat#61582, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PRKAG1
Catalog：

61582
Application：

WB
Species Reactivity：

H, M, R

Datasheet

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